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Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification BJM
Dey,Tapati Bhanja; Banerjee,Rintu.
Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by Aspergillus awamori Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (Citrus sinensis var mosambi) peels as solid substrate whereas, α-amylase was produced from A. oryzae (IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charcoal powder in a single step with 65.1%, 69.8% and 60% recoveries, respectively. Apple juice was clarified by these decolourized and partially purified enzymes. In presence of 1% polygalacturonase from mosambi peels (9.87 U/mL) and 0.4% α-amylase (899 U/mL), maximum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polygalacturonase; Α-amylase; Activated charcoal; Decolourization; Apple juice clarification.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100014
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Decolourization of textile dye effluent by non-viable biomass of Aspergillus fumigatus BABT
Kalaiarasi,K.; Lavanya,A.; Amsamani,S.; Bagyalakshmi,G..
The aim of this work was to study the decolourization of textile dye effluent by non-viable biomass of Aspergillus fumigates. The dried non-viable fungal biomass exhibited maximum dye removal at pH 7.0 with temperature of 30ºC and 3 g/l (w/v) biomass concentration, after 24 h contact time. The results showed that the non-viable biomass possessed high efficiency for dye removal from textile effluent.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus fumigatus; Decolourization; Textile effluent.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300019
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Optimization of phenoxazinone synthase production by response surface methodology and its application in Congo red decolourization Electron. J. Biotechnol.
Jia,Honghua; Gao,Zhen; Ma,Yingying; Zhong,Chao; Xie,Yingchun; Zhou,Hua; Wei,Ping.
Background: Enzymatic decolourization has been recently proposed as a promising and eco-friendly method for treatment of synthetic dye-contaminated wastewaters. However, the processes require large quantities of enzymes, attracting significant attention in developing efficient methods for mass production of multifunctional enzymes. Several methods such as response surface methodology (RSM) and orthogonal experiment have been applied to optimize the parameters in bioprocesses for enzyme production. Results: In the present study, a laccase-like enzyme, phenoxazinone synthase (PHS) originated from Streptomyces antibioticus was recombinantly expressed in Escherichia coli BL21 (DE3). The production of PHS in E. coli BL21 was optimized by response surface...
Tipo: Journal article Palavras-chave: Congo red; Decolourization; Laccase; Phenoxazinone synthase; Response surface methodology.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500002
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